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KMID : 0861020160310050015
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Korea Journal of Herbology
2016 Volume.31 No. 5 p.15 ~ p.23
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Identification of Marker Nucleotides for the Molecular Authentication of Araliae Continentalis Radix Based on the Analysis of Universal DNA Barcode, mat K and rbc L, Sequences
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Kim Wook-Jin
Yang Sun-Gyu
Choi Go-Ya
Moon Byeong-Cheol
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Abstract
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Objectives : Araliae Continentalis Radix and Angelicae Pubescentis Radix have been used as the same medicinal name Korean and Chinese traditional medicines, respectively. The authentic Araliae Continentalis Radix is described only the root of Aralia continentalis in the Korean Pharmarcopoeia. However, the dried root of Angelica biserrata, Levisticum officinale , or Heracleum moellendorffii also has been distributed adulterants of Araliae Continentalis Radix. To develop a reliable method for identifying Araliae Continentalis Radix from adulterants, we carried out the analyses of universal DNA barcode sequences.
Methods : Four plants species were collected from different habitate and nucleotide sequences of mat K and rbcL were analyzed. The species-specific sequences and phylogenetic relationship were estimated using entire sequences of two DNA barcodes, respectively.
Results : In comparative analysis of matK sequences, we were identified 104 positions of marker nucleotide for Ar. continentalis, 3 for An. biserrata , 4 for L. officinale and 8 for H. moellendorffii enough to distinguish individual species, respectively. Furthermore, we obtained marker nucleotides in rbc L at 42 positions for Ar. continentalis, 5 for An. biserrata and 2 for H. moellendorffii, but not for L. officinale . The phylogenetic tree of matK and rbcL were showed that all samples were clustered into four groups constituting homogeneous clades within the species.
Conclusions : We confirmed that species-specific marker nucleotides of mat K sequence provides distinct genetic information enough to identify four species. Therefore, we suggest that mat K gene is useful DNA barcode for discriminating authentic Araliae Continentalis Radix from inauthentic adulterants.
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KEYWORD
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Araliae Continentalis Radix, Aralia continentalis , Marker nucleotide, matK, rbc L
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